Title: Biological markers for radiation injury: A chromogenic test for irradiated Oriental fruit fly, Bactrocera philippinensis, as quarantine diagnostic kit
Authors: Teresa Yulo-Nazarea and Alejandro Q. Nato, Jr.
Technical Report Institution: Philippine Nuclear Research Institute
Catalogued by: International Nuclear Information System
Publication Date: 1997
Volume: 29
Issue: 27
Technical Report Source: PNRI-F(PR)--98001
Technical Report Reference Number: 29036447
Abstract: A biochemical marker for gamma-irradiated Oriental fruit fly, Bactrocera philippinensis (earlier designation: Dacus dorsalis) was isolated and identified in denaturing polyacrylamide gel electrophoresis (SDS-PAGE). The biomarker, Gs-protein, is the biosynthetic product of a radiosensitive gene locus of B. philippinensis. The gene appears to be sensitive to gamma radiation even at a low dose of 25 Gy as demonstrated by the loss of the Gs-protein band from SDS-PAGE gel analysis of pupal homogenates and total soluble protein fractions of irradiated larvae. Isolated Gs-protein has a tyrosinase enzyme activity capable of converting tyrosine into an intermediate product, DOPA. An apparent molecular weight of 109 kDa was calculated from SDS-PAGE preparation of the Gs-protein using high molecular weight proteins as standards.
Citation PDF URL: http://faculty.washington.edu/aqnato/pdf/1996_Nazarea_Nato_Technical_Report_Radiation_Injury_119-129.pdf